Evaluation of the biological activity of nanoemulsions fabricated by walnut oil ( <i>Juglans regia</i> L.)

Herbal medicines are currently attracting much attention as a potential cancer treatment. Juglans regia’s wide variety of anticancer actions have been demonstrated. The aim this study is evaluating the cytotoxic and antioxidant effects walnut oil (J. regia) nanoemulsion (WO-NE) on CT26 colon cell line. dimensions structure were studied by dynamic light scattering (DLS) transmission electron microscopy (TEM). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) technique was used to determine whether or not nanoemulsions had any properties. 3-[4,5-dimethylthiazol-2-yl-2-5-[diphenyltetrazolium bromide] (MTT) cells umbilical vein endothelial (HUVEC) normal measured. DPPH experiment demonstrated that manufactured ability scavenge free radicals in dose-dependent manner, with an IC50 approximately 500 µg/mL. WO-NE found be toxic both HUVEC cells, 10 ± 2.6 µg/mL for 160 7.1 after 24 h incubation. For comparison also investigated. Due their capabilities, synthesized here proposed future anti-tumour agents.